Savanah St. Clair is a third generation Cannabis grower. She has both industry and academic experience in Research and Development. Recently her research focus has been on the biomedical aspects of plant and soil science. On the cannabis industry side, she has been focusing on the benefits and challenges of micro propagation on high-potency Southern-California strains including OG Kush and Cookies. Recently, Knockout Genetics, LLC has partnered with CRTFD to create CRTFD Plant ScienceView the profile
About the talk
Professor Savanah St. Clair from Los Angeles Pierce College outlines pros and cons of scaling up and its disadvantages, tells about plant cells, stages of plant tissue culture, shares her concerns about pests, pathogens, and pesticides, and answers all your questions.
00:22 What is plant tissue culture?
01:40 Plant cell totipotency
02:40 Media components
04:11 What is micropropagation?
05:00 Stages of plant tissue culture
06:51 Different mass propagation schemes
08:51 Possibilities in cannabis
11:14 Pros and cons to scaling up
13:13 Disadvantages of scaling up
15:19 Most important factors to scaling up
16:20 Cannabis life cycle
16:53 Role of optics
17:48 Publication and research
18:50 Cannabis breeding
20:28 Process. Nursery workflow
21:36 Concerns about pests, pathogens, and pesticides
23:17 Importance of tissue culture to international business
Included in subscription
It all starts with the plant tissue culture scheme, and this is going to be growing plants in closed vessels or in test tubes and it's performing a sterile environment. So all of the work is done in a sterile laminar flow Hood which gives a positive pressure blow sterile air over the work environment in addition to that all of the plant tissue is surface sterilized. And all of the media and vessels are sterilized as well. So if you think about Pasteur's Experiment at the University of Paris or pasteurization in
the winemaking process, it's really similar to so everything is sterilized and we can grow a plant just from a single cell or from a single node finger a whole plant from that and because we're doing the surface sterilization or just taking a really small amount of tissue from the plant we can sterilize the plants for pathogens like powdery mildew or evens the stomach viruses. I can also store for a long-term. Of time with cold storage or cryogenic conditions. This whole
idea is based on plant cell totipotency. So plant cells are in acids all stem cells. If you've heard about human stem cells that can change into different types of cells different specializations. That's just like the plant cells and the most Home Depot in plant cells are going to be the Catalyst or the parenchymal cells and Siri. All of the cells are totipotent or powerful to change into other types of cells